TY - JOUR
T1 - Nonequilibrium fluctuations of a remodeling in vitro cytoskeleton
AU - Stuhrmann, Björn
AU - Soares E Silva, Marina
AU - Depken, Martin
AU - MacKintosh, Frederick C.
AU - Koenderink, Gijsje H.
PY - 2012/8/23
Y1 - 2012/8/23
N2 - Motor proteins actively contract the actin cytoskeleton of cells and thereby give rise to nonequilibrium fluctuations as well as changes in the architecture of the cytoskeleton. Here, we show, by video microrheology of a reconstituted cytoskeleton, that motors generate time-dependent nonequilibrium fluctuations, which evolve as the network is remodeled. At earlier times, the fluctuation spectrum is dominated by strong non-Gaussian fluctuations, which arise from large displacements. At later times, directed displacements are infrequent and finally disappear. We show that these effects are due to contractile coarsening of the network into large actin-myosin foci.
AB - Motor proteins actively contract the actin cytoskeleton of cells and thereby give rise to nonequilibrium fluctuations as well as changes in the architecture of the cytoskeleton. Here, we show, by video microrheology of a reconstituted cytoskeleton, that motors generate time-dependent nonequilibrium fluctuations, which evolve as the network is remodeled. At earlier times, the fluctuation spectrum is dominated by strong non-Gaussian fluctuations, which arise from large displacements. At later times, directed displacements are infrequent and finally disappear. We show that these effects are due to contractile coarsening of the network into large actin-myosin foci.
UR - http://www.scopus.com/inward/record.url?scp=84865608468&partnerID=8YFLogxK
U2 - 10.1103/PhysRevE.86.020901
DO - 10.1103/PhysRevE.86.020901
M3 - Artículo
AN - SCOPUS:84865608468
SN - 1539-3755
VL - 86
JO - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
JF - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
IS - 2
M1 - 020901
ER -