Levels of Matrix Metalloproteases in Bladder Cancer Correlate with Tumor Grade and Invasion

Bernard Davies; Jonathan Waxman; Harpret Wasan; Paul Abel; Gordon Williams; Thomas Krausz; David Neal; David Thomas; Andrew Hanby; Frances Balkwill

Levels of Matrix Metalloproteases in Bladder Cancer Correlate with Tumor Grade and Invasion

Bernard Davies, Jonathan Waxman, Harpret Wasan, Paul Abel, Gordon Williams, Thomas Krausz, David Neal, David Thomas, Andrew Hanby, Frances Balkwill

Research output: Contribution to journal › Article › peer-review

469 Scopus citations

Abstract

We have used quantitative zymography to measure levels of the type IV collagenases matrix metalloproteinase (MMP)-9 and MMP-2 in 42 biopsies of transitional cell carcinoma and in 7 biopsies of normal bladder. Mean levels of MMP-9 were significantly higher in tumor compared with normal samples (P = 0.08). Levels of MMP-9 and active MMP-2 increased with tumor grade (test for trend, P = 0.002 and P = 0.05, respectively). Levels of MMP-9 and activated MMP-2 were also higher in invasive tumors than in superficial ones (P = 0.001 and P = 0.008, respectively). In situ hybridization studies showed that the mRNAs for both MMP-2 and MMP-9 were located chiefly in the stroma rather than epithelial tumor cells and were concentrated at the interface between the two tissues.

Original language	English
Pages (from-to)	5365-5369
Number of pages	5
Journal	Cancer Research
Volume	53
Issue number	22
State	Published - Nov 15 1993
Externally published	Yes

Cite this

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title = "Levels of Matrix Metalloproteases in Bladder Cancer Correlate with Tumor Grade and Invasion",

abstract = "We have used quantitative zymography to measure levels of the type IV collagenases matrix metalloproteinase (MMP)-9 and MMP-2 in 42 biopsies of transitional cell carcinoma and in 7 biopsies of normal bladder. Mean levels of MMP-9 were significantly higher in tumor compared with normal samples (P = 0.08). Levels of MMP-9 and active MMP-2 increased with tumor grade (test for trend, P = 0.002 and P = 0.05, respectively). Levels of MMP-9 and activated MMP-2 were also higher in invasive tumors than in superficial ones (P = 0.001 and P = 0.008, respectively). In situ hybridization studies showed that the mRNAs for both MMP-2 and MMP-9 were located chiefly in the stroma rather than epithelial tumor cells and were concentrated at the interface between the two tissues.",

author = "Bernard Davies and Jonathan Waxman and Harpret Wasan and Paul Abel and Gordon Williams and Thomas Krausz and David Neal and David Thomas and Andrew Hanby and Frances Balkwill",

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T1 - Levels of Matrix Metalloproteases in Bladder Cancer Correlate with Tumor Grade and Invasion

AU - Davies, Bernard

AU - Waxman, Jonathan

AU - Wasan, Harpret

AU - Abel, Paul

AU - Williams, Gordon

AU - Krausz, Thomas

AU - Neal, David

AU - Thomas, David

AU - Hanby, Andrew

AU - Balkwill, Frances

PY - 1993/11/15

Y1 - 1993/11/15

N2 - We have used quantitative zymography to measure levels of the type IV collagenases matrix metalloproteinase (MMP)-9 and MMP-2 in 42 biopsies of transitional cell carcinoma and in 7 biopsies of normal bladder. Mean levels of MMP-9 were significantly higher in tumor compared with normal samples (P = 0.08). Levels of MMP-9 and active MMP-2 increased with tumor grade (test for trend, P = 0.002 and P = 0.05, respectively). Levels of MMP-9 and activated MMP-2 were also higher in invasive tumors than in superficial ones (P = 0.001 and P = 0.008, respectively). In situ hybridization studies showed that the mRNAs for both MMP-2 and MMP-9 were located chiefly in the stroma rather than epithelial tumor cells and were concentrated at the interface between the two tissues.

AB - We have used quantitative zymography to measure levels of the type IV collagenases matrix metalloproteinase (MMP)-9 and MMP-2 in 42 biopsies of transitional cell carcinoma and in 7 biopsies of normal bladder. Mean levels of MMP-9 were significantly higher in tumor compared with normal samples (P = 0.08). Levels of MMP-9 and active MMP-2 increased with tumor grade (test for trend, P = 0.002 and P = 0.05, respectively). Levels of MMP-9 and activated MMP-2 were also higher in invasive tumors than in superficial ones (P = 0.001 and P = 0.008, respectively). In situ hybridization studies showed that the mRNAs for both MMP-2 and MMP-9 were located chiefly in the stroma rather than epithelial tumor cells and were concentrated at the interface between the two tissues.

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M3 - Artículo

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AN - SCOPUS:0027384917

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JO - Cancer Research

JF - Cancer Research

IS - 22

ER -

Levels of Matrix Metalloproteases in Bladder Cancer Correlate with Tumor Grade and Invasion

Abstract

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