TY - JOUR
T1 - Electrochemical Monitoring of Cadmium Stress in Manihot Esculenta Crantz Using Hydrogen Peroxide as a Functional Biomarker
AU - Bustamante, Paulo Cesar
AU - Fernández, Lenys
AU - Espinoza-Montero, Patricio J.
AU - Rodríguez, Augusto
AU - Uribe, Rafael
AU - Ortega-Gallegos, Andrea
AU - Jadan, Mónica
N1 - Publisher Copyright:
© 2026 The Author(s). Published on behalf of The Electrochemical Society by IOP Publishing Limited. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License (CC BY, https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse of the work in any medium, provided the original work is properly cited.
PY - 2026/6
Y1 - 2026/6
N2 - An electrochemical method based on differential pulse voltammetry (DPV) employing a glassy carbon electrode was developed for the quantification of H2O2 in Manihot esculenta Crantz cell suspensions derived from tuber explants. Cell cultures were exposed to different concentrations of cadmium (Cd2+) for 24, 48, and 72 h. The analytical protocol exhibited a limit of detection of 10.28 μmol l−1 and a limit of quantification of 51.81 μmol l−1. Cadmium treatment induced a transient increase in H2O2 levels, consistent with an oxidative burst followed by antioxidant-mediated regulation. This behaviour reflects the dynamic activation of redox signalling pathways in cassava cells under metal stress. Overall, the findings indicate that M. esculenta Crantz may serve as a promising sedentary biomarker for cadmium contamination, providing a sensitive, reproducible, and cost-effective strategy for environmental monitoring in tropical regions.
AB - An electrochemical method based on differential pulse voltammetry (DPV) employing a glassy carbon electrode was developed for the quantification of H2O2 in Manihot esculenta Crantz cell suspensions derived from tuber explants. Cell cultures were exposed to different concentrations of cadmium (Cd2+) for 24, 48, and 72 h. The analytical protocol exhibited a limit of detection of 10.28 μmol l−1 and a limit of quantification of 51.81 μmol l−1. Cadmium treatment induced a transient increase in H2O2 levels, consistent with an oxidative burst followed by antioxidant-mediated regulation. This behaviour reflects the dynamic activation of redox signalling pathways in cassava cells under metal stress. Overall, the findings indicate that M. esculenta Crantz may serve as a promising sedentary biomarker for cadmium contamination, providing a sensitive, reproducible, and cost-effective strategy for environmental monitoring in tropical regions.
KW - cadmium stress
KW - electrochemical sensing
KW - environmental biomonitoring
KW - hydrogen peroxide
KW - manihot esculenta
UR - https://www.scopus.com/pages/publications/105038640432
U2 - 10.1149/2754-2726/ae670f
DO - 10.1149/2754-2726/ae670f
M3 - Artículo
AN - SCOPUS:105038640432
SN - 2754-2726
VL - 5
JO - ECS Sensors Plus
JF - ECS Sensors Plus
IS - 2
M1 - 020603
ER -