A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Tanya Barrett; Tao Xie; Yulan Piao; Ora Dillon-Carter; George J. Kargul; Meng K. Lim; Francis J. Chrest; Robert Wersto; Daniel L. Rowley; Magdalena Juhaszova; Li Zhou; Marquis P. Vawter; Kevin G. Becker; Christopher Cheadle; William H. Wood; Una D. McCann; William J. Freed; Minoru S. Ko; George A. Ricaurte; David M. Donovan

doi:10.1006/nbdi.2001.0423

A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Tanya Barrett
, Tao Xie
, Yulan Piao
, Ora Dillon-Carter
, George J. Kargul
, Meng K. Lim
, Francis J. Chrest
, Robert Wersto
, Daniel L. Rowley
, Magdalena Juhaszova
, Li Zhou
, Marquis P. Vawter
, Kevin G. Becker
, Christopher Cheadle
, William H. Wood
, Una D. McCann
, William J. Freed
, Minoru S. Ko
, George A. Ricaurte
, David M. Donovan

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

Original language	English
Pages (from-to)	822-833
Number of pages	12
Journal	Neurobiology of Disease
Volume	8
Issue number	5
DOIs	https://doi.org/10.1006/nbdi.2001.0423
State	Published - 2001
Externally published	Yes

Keywords

Development
Dopamine
Gene expression profile
Methamphetamine
cDNA library
cDNA microarray

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10.1006/nbdi.2001.0423

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Barrett, T., Xie, T., Piao, Y., Dillon-Carter, O., Kargul, G. J., Lim, M. K., Chrest, F. J., Wersto, R., Rowley, D. L., Juhaszova, M., Zhou, L., Vawter, M. P., Becker, K. G., Cheadle, C., Wood, W. H., McCann, U. D., Freed, W. J., Ko, M. S., Ricaurte, G. A., & Donovan, D. M. (2001). A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity. Neurobiology of Disease, 8(5), 822-833. https://doi.org/10.1006/nbdi.2001.0423

@article{b75beca64e014d96b32ecbacc9cda49f,

title = "A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity",

abstract = "Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.",

keywords = "Development, Dopamine, Gene expression profile, Methamphetamine, cDNA library, cDNA microarray",

author = "Tanya Barrett and Tao Xie and Yulan Piao and Ora Dillon-Carter and Kargul, \{George J.\} and Lim, \{Meng K.\} and Chrest, \{Francis J.\} and Robert Wersto and Rowley, \{Daniel L.\} and Magdalena Juhaszova and Li Zhou and Vawter, \{Marquis P.\} and Becker, \{Kevin G.\} and Christopher Cheadle and Wood, \{William H.\} and McCann, \{Una D.\} and Freed, \{William J.\} and Ko, \{Minoru S.\} and Ricaurte, \{George A.\} and Donovan, \{David M.\}",

year = "2001",

doi = "10.1006/nbdi.2001.0423",

language = "Ingl{\'e}s",

volume = "8",

pages = "822--833",

journal = "Neurobiology of Disease",

issn = "0969-9961",

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}

Barrett, T, Xie, T, Piao, Y, Dillon-Carter, O, Kargul, GJ, Lim, MK, Chrest, FJ, Wersto, R, Rowley, DL, Juhaszova, M, Zhou, L, Vawter, MP, Becker, KG, Cheadle, C, Wood, WH, McCann, UD, Freed, WJ, Ko, MS, Ricaurte, GA & Donovan, DM 2001, 'A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity', Neurobiology of Disease, vol. 8, no. 5, pp. 822-833. https://doi.org/10.1006/nbdi.2001.0423

TY - JOUR

T1 - A murine dopamine neuron-specific cDNA library and microarray

T2 - Increased COX1 expression during methamphetamine neurotoxicity

AU - Barrett, Tanya

AU - Xie, Tao

AU - Piao, Yulan

AU - Dillon-Carter, Ora

AU - Kargul, George J.

AU - Lim, Meng K.

AU - Chrest, Francis J.

AU - Wersto, Robert

AU - Rowley, Daniel L.

AU - Juhaszova, Magdalena

AU - Zhou, Li

AU - Vawter, Marquis P.

AU - Becker, Kevin G.

AU - Cheadle, Christopher

AU - Wood, William H.

AU - McCann, Una D.

AU - Freed, William J.

AU - Ko, Minoru S.

AU - Ricaurte, George A.

AU - Donovan, David M.

PY - 2001

Y1 - 2001

N2 - Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

AB - Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

KW - Development

KW - Dopamine

KW - Gene expression profile

KW - Methamphetamine

KW - cDNA library

KW - cDNA microarray

UR - https://www.scopus.com/pages/publications/0035173005

U2 - 10.1006/nbdi.2001.0423

DO - 10.1006/nbdi.2001.0423

M3 - Artículo

AN - SCOPUS:0035173005

SN - 0969-9961

VL - 8

SP - 822

EP - 833

JO - Neurobiology of Disease

JF - Neurobiology of Disease

IS - 5

ER -

A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Abstract

Keywords

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