A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Tanya Barrett; Tao Xie; Yulan Piao; Ora Dillon-Carter; George J. Kargul; Meng K. Lim; Francis J. Chrest; Robert Wersto; Daniel L. Rowley; Magdalena Juhaszova; Li Zhou; Marquis P. Vawter; Kevin G. Becker; Christopher Cheadle; William H. Wood; Una D. McCann; William J. Freed; Minoru S. Ko; George A. Ricaurte; David M. Donovan

doi:10.1006/nbdi.2001.0423

A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Tanya Barrett, Tao Xie, Yulan Piao, Ora Dillon-Carter, George J. Kargul, Meng K. Lim, Francis J. Chrest, Robert Wersto, Daniel L. Rowley, Magdalena Juhaszova, Li Zhou, Marquis P. Vawter, Kevin G. Becker, Christopher Cheadle, William H. Wood, Una D. McCann, William J. Freed, Minoru S. Ko, George A. Ricaurte, David M. Donovan

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

Original language	English
Pages (from-to)	822-833
Number of pages	12
Journal	Neurobiology of Disease
Volume	8
Issue number	5
DOIs	https://doi.org/10.1006/nbdi.2001.0423
State	Published - 2001
Externally published	Yes

Keywords

cDNA library
cDNA microarray
Development
Dopamine
Gene expression profile
Methamphetamine

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10.1006/nbdi.2001.0423

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Barrett, T., Xie, T., Piao, Y., Dillon-Carter, O., Kargul, G. J., Lim, M. K., Chrest, F. J., Wersto, R., Rowley, D. L., Juhaszova, M., Zhou, L., Vawter, M. P., Becker, K. G., Cheadle, C., Wood, W. H., McCann, U. D., Freed, W. J., Ko, M. S., Ricaurte, G. A., & Donovan, D. M. (2001). A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity. Neurobiology of Disease, 8(5), 822-833. https://doi.org/10.1006/nbdi.2001.0423

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title = "A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity",

abstract = "Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.",

keywords = "cDNA library, cDNA microarray, Development, Dopamine, Gene expression profile, Methamphetamine",

author = "Tanya Barrett and Tao Xie and Yulan Piao and Ora Dillon-Carter and Kargul, \{George J.\} and Lim, \{Meng K.\} and Chrest, \{Francis J.\} and Robert Wersto and Rowley, \{Daniel L.\} and Magdalena Juhaszova and Li Zhou and Vawter, \{Marquis P.\} and Becker, \{Kevin G.\} and Christopher Cheadle and Wood, \{William H.\} and McCann, \{Una D.\} and Freed, \{William J.\} and Ko, \{Minoru S.\} and Ricaurte, \{George A.\} and Donovan, \{David M.\}",

year = "2001",

doi = "10.1006/nbdi.2001.0423",

language = "Ingl{\'e}s",

volume = "8",

pages = "822--833",

journal = "Neurobiology of Disease",

issn = "0969-9961",

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Barrett, T, Xie, T, Piao, Y, Dillon-Carter, O, Kargul, GJ, Lim, MK, Chrest, FJ, Wersto, R, Rowley, DL, Juhaszova, M, Zhou, L, Vawter, MP, Becker, KG, Cheadle, C, Wood, WH, McCann, UD, Freed, WJ, Ko, MS, Ricaurte, GA & Donovan, DM 2001, 'A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity', Neurobiology of Disease, vol. 8, no. 5, pp. 822-833. https://doi.org/10.1006/nbdi.2001.0423

TY - JOUR

T1 - A murine dopamine neuron-specific cDNA library and microarray

T2 - Increased COX1 expression during methamphetamine neurotoxicity

AU - Barrett, Tanya

AU - Xie, Tao

AU - Piao, Yulan

AU - Dillon-Carter, Ora

AU - Kargul, George J.

AU - Lim, Meng K.

AU - Chrest, Francis J.

AU - Wersto, Robert

AU - Rowley, Daniel L.

AU - Juhaszova, Magdalena

AU - Zhou, Li

AU - Vawter, Marquis P.

AU - Becker, Kevin G.

AU - Cheadle, Christopher

AU - Wood, William H.

AU - McCann, Una D.

AU - Freed, William J.

AU - Ko, Minoru S.

AU - Ricaurte, George A.

AU - Donovan, David M.

PY - 2001

Y1 - 2001

N2 - Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

AB - Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

KW - cDNA library

KW - cDNA microarray

KW - Development

KW - Dopamine

KW - Gene expression profile

KW - Methamphetamine

UR - https://www.scopus.com/pages/publications/0035173005

U2 - 10.1006/nbdi.2001.0423

DO - 10.1006/nbdi.2001.0423

M3 - Artículo

AN - SCOPUS:0035173005

SN - 0969-9961

VL - 8

SP - 822

EP - 833

JO - Neurobiology of Disease

JF - Neurobiology of Disease

IS - 5

ER -

A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Abstract

Keywords

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