TY - JOUR
T1 - A genetically encoded photosensitizer
AU - Bulina, Maria E.
AU - Chudakov, Dmitriy M.
AU - Britanova, Olga V.
AU - Yanushevich, Yurii G.
AU - Staroverov, Dmitry B.
AU - Chepurnykh, Tatyana V.
AU - Merzlyak, Ekaterina M.
AU - Shkrob, Maria A.
AU - Lukyanov, Sergey
AU - Lukyanov, Konstantin A.
PY - 2006/1
Y1 - 2006/1
N2 - Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation1. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, a homolog of green fluorescent protein (GFP). KillerRed generates ROS upon irradiation with green light. Whereas known photosensitizers must be added to living systems exogenously, KillerRed is fully genetically encoded. We demonstrate the utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to β-galactosidase and phospholipase Cδ1 pleckstrin homology domain.
AB - Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation1. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, a homolog of green fluorescent protein (GFP). KillerRed generates ROS upon irradiation with green light. Whereas known photosensitizers must be added to living systems exogenously, KillerRed is fully genetically encoded. We demonstrate the utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to β-galactosidase and phospholipase Cδ1 pleckstrin homology domain.
UR - http://www.scopus.com/inward/record.url?scp=30544443562&partnerID=8YFLogxK
U2 - 10.1038/nbt1175
DO - 10.1038/nbt1175
M3 - Artículo
C2 - 16369538
AN - SCOPUS:30544443562
SN - 1087-0156
VL - 24
SP - 95
EP - 99
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 1
ER -